Reliability of IFCC method for lactate dehydrogenase measurement in lithium-heparin plasma samples.

نویسندگان

  • Ileana Herzum
  • Robert Bünder
  • Harald Renz
  • Hans Günther Wahl
چکیده

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for measuring LD in heparin plasma is necessary. We have shown that in contrast to the nonevacuated heparin tubes from Sarstedt (5 ), the evacuated lithium-heparin tubes from Becton Dickinson cause a high frequency of duplicate errors in the IFCC method for LD. Increased attention to the preanalytical steps (additional mixing before centrifuging) and adapting the analytical procedure by introdu...

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IFCC method for lactate dehydrogenase measurement in heparin plasma is unreliable.

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samples and hemolysis can be responsible for LD measurements with poor precision in addition to higher LD activity values (7, 8). To investigate these effects, we performed LD measurements with serum-gel (Sarstedt Monovette prod. no. 02.1388), lithium-heparin-gel (same as given above), and lithium-heparin tubes (Sarstedt Monovette prod. no. 01.1604.400). Blood samples were collected from the sa...

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Commutable calibrator with value assigned by the IFCC reference procedure to harmonize serum lactate dehydrogenase activity results measured by 2 different methods.

BACKGROUND The availability of commutable calibrator materials may ease considerably the task of harmonizing assay results and ensuring their traceability to reference procedures. We sought to verify the commutability of potential calibrator materials and evaluate their effectiveness in harmonizing LDH results by 2 measurement methods. METHODS We measured LDH in 109 serum samples and 31 mater...

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Comparison of hematologic values in blood samples with lithium heparin or dipotassium ethylenediaminetetraacetic acid anticoagulants in Hispaniolan Amazon parrots (Amazona ventralis).

Blood samples were collected from 20 Hispaniolan Amazon parrots (Amazona ventralis) and were divided into tubes that contained dipotassium ethylenediaminetetraacetic acid (K2EDTA) and lithium heparin. Complete blood cell counts were determined in each sample within 2 hours of collection. The level of agreement in results was moderate for plasma protein, packed cell volume (PCV), and leukocyte, ...

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عنوان ژورنال:
  • Clinical chemistry

دوره 49 12  شماره 

صفحات  -

تاریخ انتشار 2003